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Electrospray ionization quadrupole time-of-flight tandem mass spectromteric analysis of hexamethylenediamine-modified maltodextrin and dextran

Domenii publicaţii > Chimie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: E.Sisu, W.T.E.Bosker,W.Norde,T.M.Slaghek,J.W.Timmermans,J.P.Katalinic,M.A.Cohen-Stuart,A.Zamfir

Editorial: John Wiley & Sons, Rapid Commun.Mass Spectrom, 20, p.209-218, 2006.


A combined methodology for obtaining at the preparative scale and characterization by nanoelectrospray
ionization (nanoESI) quadrupole time-of-flight (QTOF) mass spectrometry (MS) and tandem
MS (MS/MS) of linear polysaccharides modified at the reducing end is presented. Two
polydisperse maltodextrins (1000 and 3000 Da) and a high molecular weight polydisperse dextran
(6000 Da) were coupled with hexamethylenediamine (HMD). The coupling products were analyzed
by nanoESI-QTOF-MS in the positive ion mode and MS/MS using collision-induced dissociation
(CID) at low energies. In the HMD-M1000 mixture, the polysaccharide chains containing from 2 to
8 Glc residues were detected, while in HMD-M3000 we identified a complete series of chains containing
from 8 to 21 Glc moieties. The employed ESI conditions enhanced the detection of chains
with up to 46 Glc residues in the HMD-D6000 sample. By optimized MS/MS, HMD-modified polysaccharides
of 3, 4, 5, 12 and 46 degrees of polymerization yielded product ion spectra exhibiting the
whole set of Y- and B-fragment ions. The MS structural data were obtained within a few minutes of
signal acquisition, with a sample consumption situating the analysis sensitivity in the picomolar

Cuvinte cheie: nanoESI,QTOF,maltodextrines,dextran // nanoESI,QTOF,maltodextrines,dextran