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Circulating Endothelial Progenitor Cell and platelet microparticle impact on platelet activation in hypertension associated with hypercholesterolemia

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Nicoleta Alexandru, Doina Popov, Emanuel Dragan, Eugen Andrei, Adriana Georgescu

Editorial: Shree Ram Singh, National Cancer Institute, USA, PLOS ONE (eISSN-1932-6203) , PLoS ONE, 8(1), p.e52058, 2013.


Aim: The purpose of this project was to evaluate the influence of circulating endothelial progenitor cells (EPCs) and platelet microparticles (PMPs) on blood platelet function in experimental hypertension associated with hypercholesterolemia.

Methods: Golden Syrian hamsters were divided in six groups: (i) control, C; (ii) hypertensive-hypercholesterolemic, HH; (iii)‘prevention’, HHin-EPCs, HH animals fed a HH diet and treated with EPCs; (iv) ‘regression’, HHfin-EPCs, HH treated with EPCs after HH feeding; (v) HH treated with PMPs, HH-PMPs, and (vi) HH treated with EPCs and PMPs, HH-EPCs-PMPs.

Results: Compared to HH group, the platelets from HHin-EPCs and HHfin-EPCs groups showed a reduction of: (i) activation,reflected by decreased integrin 3b, FAK, PI3K, src protein expression; (ii) secreted molecules as: SDF-1, MCP-1, RANTES, VEGF,PF4, PDGF and (iii) expression of pro-inflammatory molecules as: SDF-1, MCP-1, RANTES, IL-6, IL-1b; TFPI secretion was increased. Compared to HH group, platelets of HH-PMPs group showed increased activation, molecules release and proteins
expression. Compared to HH-PMPs group the combination EPCs with PMPs treatment induced a decrease of all investigated
platelet molecules, however not comparable with that recorded when EPC individual treatment was applied.

Conclusion: EPCs have the ability to reduce platelet activation and to modulate their pro-inflammatory and antithrombogenic properties in hypertension associated with hypercholesterolemia. Although, PMPs have several beneficial effects in combination with EPCs, these did not improve the EPC effects. These findings reveal a new biological role of
circulating EPCs in platelet function regulation, and may contribute to understand their cross talk, and the mechanisms of atherosclerosis.

Cuvinte cheie: Celule endoteliale progenitoare, plachete,microparticule, hipertensiune, hipercolesterolemie // EPCs, platelets, microparticles, hypertension, hypercholesterolemia

URL:; doi:10.1371/journal.pone.0052058