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Calcium signaling mediates the response to cadmium toxicity in Saccharomyces cerevisiae cells

Domenii publicaţii > Biologie + Tipuri publicaţii > Articol în revistã ştiinţificã

Autori: Lavinia L. Ruta, Valentina C. Popa, Ioana Nicolau, Andrei F. Danet, Virgil Iordache, Aurora D. Neagoe, Ileana C. Farcasanu

Editorial: FEBS Letters, 588, p.3202-3212, 2014.


The involvement of Ca2+ in the response to high Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, and Hg2+ was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca2+ when exposed to Cd2+, and to a lesser extent to Cu2+, but not to Mn2+, Co2+, Ni2+, Zn2+, or Hg2+. The response to high Cd2+ depended mainly on external Ca2+ (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca2+ (released into the cytosol through the Yvc1p channel). The adaptation to high Cd2+ was influenced by perturbations in Ca2+ homeostasis. Thus, the tolerance to Cd2+ often correlated with sharp Cd2+-induced cytosolic Ca2+ pulses, while the Cd2+ sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca2+.

Cuvinte cheie: Heavy metal stress Cadmium Calcium signaling Aequorin Saccharomyces cerevisiae