Biofilms are bacterial colonies enveloped in a matrix of extracellular polymeric secretions. Confocal scanning laser microscopy has been used in conjunction with different image analysis techniques to investigate the structure of biofilms. A major goal is to reconstitute the three-dimensional structure of biofilms, and compute or estimate the biovolumes. Our previous research focused on the utilization of remote sensing techniques and Geographical

The unique geochemical coupling of organic molecules and mineral CaCO3provides a fluorescence signature detectable using conventional confocal scanning laser microscopy (CSLM). The surface microbial mats of open-water marine stromatolites (Bahamas) exist in a continuum of states ranging from a Type 1 (i.e., nonlithifying) to Type 2 (i.e., lithified micritic laminae present) to Type 3 (i.e., fused grain layer). An approach was developed here, that