Reconstruction and computation of microscale biovolumes using geographical information systems: potential difficulties
Biofilms are bacterial colonies enveloped in a matrix of extracellular polymeric secretions. Confocal scanning laser microscopy has been used in conjunction with different image analysis techniques to investigate the structure of biofilms. A major goal is to reconstitute the three-dimensional structure of biofilms, and compute or estimate the biovolumes. Our previous research focused on the utilization of remote sensing techniques and Geographical
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