The less active Gentamicin C1 have been selectively removed from the biosynthetic mixture containing Gentamicins C1, C1a, C2 and C2a by facilitated pertraction with D2EHPA dissolved in dichloromethane as the liquid membrane U-shaped pertraction cell. The pertraction has been analyzed by means of initial and final mass flows, permeability and selectivity factors. The main factors, which control the pertraction selectivity, were identified to be the

Cinnamic acid can be selectively separated from p-methoxycinnamic acid by facilitated pertraction with Amberlite LA-2 dissolved in dichloromethane, using a U-shaped pertraction cell which allows obtaining the free liquid membrane. The pertraction has been analyzed by means of initial and final mass flows, permeability and selectivity factors. The main factors which control the pertraction selectivity were identified to be the pH-gradient between

The experiments on reactive extraction of gentamicins C1, C1a, C2 and C2a with D2EHPA dissolved in dichloromethane indicated the possibility to selectively separate the gentamicin C1 from the mixture obtained by biosynthesis. The selectivity of its extraction is positively influenced by increasing the pH-value of aqueous solution and by using a D2EHPA concentration in organic phase near the value needed for an equimolecular ratio between the extractant

The mixing time and oxygen mass transfer coefficient represent two of the most important parameters implied on the design and operation of the bioreactors. In this paper, the influences of the main characteristics of bioreactor and of fungus broths (P. chrysogenum, free mycelia and mycelial aggregates) on mixing efficiency and oxygen mass transfer are studied. By means of the experimental data, some mathematical correlations describing the influences

The selective separation of Penicillin V from phenoxyacetic acid using extraction and transport through liquid membranes consisting of 1,2-dichloroethane and Amberlite LA-2 as carrier was studied. The selective separation is the result of the difference between the acidity and hydrophobicity of the two compounds. The experimental data indicated the decisive in¯uences of pH (difference between the feed and stripping phases), carrier concentration

The method of reactive extraction with di-(2-ethylhexyl)phosphoric acid (D2EHPA) for the separation of a range of amino acids is studied. The results obtained on the individual reactive extraction indicated the possibility of the amino acids selective separation as a function of the pH value of aqueous solution and the acidic or basic character of each amino acid. Thus, using multistage extraction, the total separation of the following amino acids

The mixing time is one of the most useful criterion for mixing intensity of fermentation broths and for scale-up of biosynthesis processes. This parameter value depends mainly on the rheological properties of the broths, biomass concentration and morphology, mixing system characteristics and fermentation conditions. For quantifying the influence of these factors on mixing efficiency for stirred bioreactors, these studies were carried out for non-aerated

Pertraction through liquid membranes is one of the most recent liquid-liquid extraction techniques that could be applied for separation of biosynthetic products. In this paper, the facilitated pertraction has been used for selective separation of citric, malic and succinic acids from a mixture. The separation equipment includes a U-shaped cell containing 1,2-dichloroethane as liquid membrane and Amberlite LA-2 as carrier. The experimental data indicated

The oxygen mass transfer represents the most important parameter implied on the design and operation of mixing-sparging equipment of the bioreactors. It can be described and analyzed by means of mass transfer coefficient, kla. The kla values are affected by a lot of factors, such as geometrical and operational characteristics of the vessels, media composition, type, concentration and microorganisms morphology, biocatalysts properties. The aim of