Scopul nostru este sprijinirea şi promovarea cercetării ştiinţifice şi facilitarea comunicării între cercetătorii români din întreaga lume.
ABSTRACT: Purpose of the paper was the optimization of mobile phase in natrium chloride gradient in the chromatographic separation of butyrylcholinesterase from human plasma. Materials/Methods. Butyrylcholinesterase (BuChE) was isolated from human plasma using a diethylamminoethyl-cellulose column, by elution with 0.02M acetate buffer pH=4.0, gradually increasing NaCl percentage from 10% to 80%. The procedure lasted approximately 28 days. Results.Read more
Abstract The objectives of this study were to develop and validate a method for the simultaneous HPLC-UV determination of obidoxime (OBI), scopolamine (SCA), Atropine (ATR) and imipramine (I) in an aqueous antidote solution. An ion-pair mechanism with DAD (diode array detector) detection was used to quantitatively determine the four drugs. The proposed HPLC method uses a Kromasil 100-5C8 (250x4.6mm) column, gradient elution, at 25°C column temperature.Read more
Abstract Manuscript received: December 2014 The purpose of this study was to evaluate the in vitro release kinetics of Na3CaDTPA, a well-known and widely-used decorporation agent, from a topical microemulsion-gel formulation in comparison with a conventional gel. For the preparation of hydrogels, Carbopol 934 polymer was used. Release studies were conducted using both hydrophobic (polycarbonate) and hydrophilic (mixed cellulose esters) membranes.Read more
The study presents the development and validation of a simple HPLC method for the simultaneous determination of metformin (MTF) and gliclazide (GCZ) in the presence of glibenclamide, in human plasma, for the clinical monitoring of MTF and GCZ after oral administration or for bioequivalence studies. Ion-pair separation followed by UV detection performed on deproteinised plasma samples was chosen for the determination of metformin and gliclazide. TheRead more
The paper presents the layout and the technological steps for an interdigitated integrated capacitor used for gases detection. Silicon micromachining technology is applied for manufacturing the sensor substrate. The sensitive layer used is phthalocyanine (Pc) deposed by evaporation technique under high vacuum. The phthalocyanine derivatives are obtained by the same deposition technique. Considering the different sensitivities of phthalocyanines derivatives,Read more