The stone crayfish, Austropotamobius torrentium, is one of the oldest freshwater crayfish species in Europe. Most Carpathian populations are in Romania, with a distribution clustered in 2 compact metapopulations in the western part of the country. Our goal was to understand if this pattern is the result of a coincidence or a complex set of restrictive circumstances. Romania is an ideal place to analyze crayfish distributions because they have not

The increasing availability of high resolution imagery has triggered the need for automated image analysis techniques, with reduced human intervention and reproducible analysis procedures. The knowledge gained in the past might be of use to achieving this goal, if systematically organized into libraries which would guide the image analysis procedure. In this study we aimed at evaluating the variability of digital classifications carried out by three